![]() Please use the navigation bar at left to explore, and note that pressing the question mark button in any window in the FlowJo application will launch a web browser and access a help page relevant to that window.Īs a note, we are pleased to be able to frequently update FlowJo to provide new features and analysis capabilities. These files are read using FlowJo software regardless of what kind of flow cytometer was used to collect the data. Files produced by the flow cytometer are all written in a Flow Cytometry Standard file format, with a. They modify the attributes of the current graph (or currently-selected gate.) The ‘Statistic’ selection is the statistics that will be applied to. FlowJo is a common software package used to analyze flow cytometry data. When you click the Options disclosure triangle in the kinetics window, the options shown below appear. Histogram overlays have the additional option of adding an offset that produces a 3D-like plot of the multiple histograms. Our web manual is a search-able set of pages which explains the many features and functions in FlowJo. The graph specifications control what metric is used on the Y-axis, and whether smoothing will be applied. Double-click the gate to open a graph window of the child population. The workspace is saved as a FlowJo document on your hard disk when you reopen the document, you will see your analysis as it was when it was last saved. Change the Y- axis to Histogram Create a wide ranged gate on the histogram, excluding events near 0. The environment is presented as the Workspace, which contains a list of loaded samples (experimental data), statistics, gates, and other analyses, as well as tabular and graphical layouts. (The exception to this rule is if you open the parent graph window using the open parent (up-arrow) button in a graph window, this function opens. The next time you open that subset for display, FlowJo will show you the same graph setup. Welcome to FlowJo, a software application with an integrated environment for viewing and analyzing flow cytometric data. When you close a graph window, FlowJo remembers exactly what you displayed (axes, options, and graph type).
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